We collected 75 immature Pardosa milvina and maintained them in a laboratory until death or maturity to determine whether P. milvina in our population were harboring endoparasites. Nine mermithid nematodes emerged from P. milvina hosts, with each nematode emerging from a separate spider. One dipteran parasite, an acrocerid, emerged from P. milvina. This study provides the first published record of nematodes emerging from P. milvina and documents an additional record of acrocerid parasitism of P. milvina.